Hibernation 28-37 mammals.fh1

Seasonal timing and pre-hibernation
32 fattening in breeding and non-breeding
European Ground Squirrels
EVA MILLESI, ANITA DIVJAK & ANNA STRAUSSDepartment of Behavioural Biology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria; eva.millesi@univie.ac.at Abstract
We compared seasonal timing, body mass changes and plasma progesterone secretion in
breeding and non-breeding female European Ground Squirrels. One group of females could mate normally in spring, other individuals had no access to males. The ground squirrels were born and kept in semi-natural conditions in outdoor enclosures. Females were captured at weekly intervals, weighed and reproductive status was determined. The results showed that the course and extent of prehibernation fattening were similar in non-reproductive and postreproductive females. In non-breeding females, however, fattening occurred earlier, resulting in immergence into the hibernacula 4–6 weeks ahead of the annual schedule. Body mass at immergence was similar in both groups. Progesterone secretion increased in both groups prior to pre-hibernation fattening. Before hibernation onset, progesterone secretion decreased to baseline levels in al females. In general, our results demonstrate that the lack of reproductive effort in female ground squirrels led to a shift in the seasonal timing of fattening, earlier immergence and probably an extended hibernation period.
Introduction
Similar to many other hibernating ground squirrel species, female European Ground Squirrels
(Spermophilus citel us) mate shortly after spring emergence (reviewed in Michener 1984; Mil esi et al. 1999a), give birth after 4 weeks of gestation and lactate their offspring for 4–6 weeks. After weaning, females use the remaining part of their active season to prepare for hibernation.
European Ground Squirrels rely solely on their body fat reserves during winter (Millesi et al.
1998). Thus, the postreproductive period is characterised by a rapid body mass increase during prehibernation fattening (Mil esi et al. 1999b). Adult females usual y start hibernation in early August, fol owed by adult males in late August, and juveniles in late September. Female S. citel us start breeding after their first hibernation and can live for up to six years in the field (Hoffmann et al. 2003). Hence, a non-reproductive season may on the one hand reduce lifetime reproductive success, but on the other hand, could enhance future reproductive output by improving a female’s Lovegrove, B.G. & McKechnie, A.E. (eds) Hypometabolism in animals: hibernation, torpor and cryobiology.
University of KwaZulu-Natal, Pietermaritzburg.
condition in the subsequent spring, as has been shown in alpine marmots (Hackländer & Arnold 1999). In this study we compared the duration of the active season, timing and course of pre- hibernation fattening, immergence into the hibernaculum, body mass changes and progesterone secretion in breeding and non-breeding female European Ground Squirrels kept in semi-natural conditions. Considering the energetic costs of reproductive effort, we expected the non-breeding females to increase body mass throughout the season, entering hibernation with a higher body mass than reproductive individuals. Progesterone secretion has been found to increase during late lactation and remain elevated until shortly before hibernation, indicating an active luteal phase during the postreproductive period (Strauss et al. unpubl. data). In fact, active corpora lutea have been found in females shortly after weaning and during prehibernation fattening (Mil esi et al. unpubl. data). In this study we compared the course of plasma progesterone secretion between breeding and non-breeding females. Owing to the absence of pregnancy and lactation we expected lower progesterone levels in non-pregnant compared to pregnant females and, similar to the postreproductive progesterone peak mentioned above, elevated progesterone secretion in non-breeding females during an active luteal phase following ovulation.
Materials and methods
Animals, study area and capture techniques
Female European Ground Squirrels (Spermophilus citel us), living under semi-natural conditions
in outdoor enclosures built within the habitat of the species north of Vienna (Austria), were investigated from vernal emergence until the onset of hibernation. In the largest enclosure (240 m2) al males were removed prior to female emergence. Hence, none of these females mated or showed signs of gestation or lactation (n = 10). In two other enclosures (160 m2 each), females had access to males and reproduced successfully (n = 8). Individual density was similar in all enclosures. The density in the enclosures was higher than that of free-living populations in close distance but corresponded to that in preferred parts during high-density periods (Hoffmann et al. 2003). Al animals were permanently marked with subcutaneous transponder chips injected in the neck region (PIT-tag, Datamars Comp.). For recognition from a distance, the fur was painted with commercial hair dye in a unique pattern. Females were captured at weekly intervals using Tomahawk live traps baited with peanut butter. At each capture, animals were weighed (± 0.5 g, Sartorius laboratory scale) and reproductive status was determined. To determine vernal emergence dates of individual females, all burrows in the enclosures were controlled at daily intervals between mid-February until al ground squirrels had started aboveground activity.
Opened burrows could easily be detected by digging traces. The animals could be identified by their fur marks and were captured within one or two days. Gestation and lactation were determined by examining body-mass changes and teat development. Pregnant females were recognised by dark-pigmented teats and rapid body mass increase. Parturition was detected by an abrupt drop of body mass at the expected time. During lactation, females had large, light-pigmented teats, and milk secretion could be induced. Weaning had occurred when the teats were dried up and no further signs of lactation were determined. Pre-hibernation fattening was defined by mass increases < 1 g/d, excluding the gestation period in breeding females (Mil esi Seasonal timing and pre-hibernation fattening in breeding and non-breeding European Ground Squirrels Immergence date was determined by the last sighting of an individual female during summer. In addition, the burrows of the experimental females were frequently checked and closed burrows of animals that had terminated above-ground activity could be identified.
All animal procedures were performed in accordance with Austrian legal requirements for animal experimentation and were approved by the Austrian Committee for Animal Research of the Federal Ministry of Agriculture, Forestry, Environment and Water Management Hormone assay
Blood samples (150–200 l) were taken from the femoral vein at weekly intervals. The blood was
collected in heparinised capillaries and centrifuged in the field. Plasma was stored at -20°C until assayed for hormone concentrations. Progesterone levels were analysed in duplicate after diethylether extraction with a biotin-streptavidin enzyme immunoassay (Palme & Möstl 1994).
Intra- and inter-assay coefficients of variation were 10.6% and 15.1%.
Results
Body mass in females that had mated increased during pregnancy in April (Figure 1). At
parturition, body mass dropped and did not increase until weaning in late June. Thereafter, prehibernation fattening occurred, defined as a period of rapid mass increase (Figure 1). Fattening started in late June and the body mass increase levelled off by mid-July. In non-breeding individuals, body mass started to increase rapidly in mid-May. In these individuals fattening started four weeks earlier than in breeding females (Figure 1). The duration of the fattening period did not differ significantly between breeding and non-breeding females (breeding: 30.3 ± 9.9 d, n = 8; non-breeding: 29 ± 7.1 d, n = 10; Mann-Whitney U test, p = 0.8). Hence, non- breeding individuals reached their maximum body mass earlier in the season than breeding females. Daily mass increase rates during fattening were similar in both female groups (breeding: 3.5 ± 1.0 g/d, n = 8; non-breeding: 3.7 ± 1.2 g/d, n = 10; Mann-Whitney U test, p = 0.7).
The differences in the timing of the fattening period were reflected in the termination of above-ground activity. Non-breeding females immerged about one month earlier than individuals that had raised a litter (Table 1). Some of the non-breeding females even went into Figure 1.
Body mass changes in
reproductive and non-reproductive EuropeanGround Squirrels. Means in 2-week intervals ± SDare shown (reproductive:n = 8,8,8,8,8,8,8,8,6,1;non-reproductive:n = 10,10,10,10,10,4,2).
their hibernacula by mid-June. As spring emergence dates did not differ between the two groups, the active period in non-breeding females was about 4 weeks shorter than in breeding ones (84 d ± 8 vs 115 d ± 17, p < 0.001, n = 10/8). Neither body mass at vernal emergence nor shortly before immergence differed significantly between the two female groups (Table 1).
Progesterone secretion patterns differed between the two groups in that during April, levels were baseline in non-reproductive individuals, but elevated in females that had mated and become pregnant (Mann-Whitney U test, p < 0.01) (Figure 2). Thereafter, plasma progesterone secretion increased rapidly in non-breeding females until levels peaked in late May and remained elevated until mid-June. In breeding females, progesterone levels decreased during parturition and early lactation, increased thereafter and were elevated from late May until late June. Pro- gesterone secretion declined earlier in non-breeding females but with the same rate as in breeding individuals and reached baseline levels in both groups prior to immergence (Figure 2). Discussion
Unmated European Ground Squirrels could use additional time for growth and fattening. All
experimental females were older than two years and hence definitely adult. Thus, their pronounced mass increase was entirely due to fattening. Prehibernatory fattening started earlier in non-breeding females than in individuals that had raised a litter. Maximum annual body mass was reached by early July in non-breeding females, about four weeks prior to breeding individuals. Daily mass increase rates during fattening did not differ in non-reproductive and postreproductive females. Accordingly, immergence body mass was similar in both groups.
Nunes et al. (2002) showed that juvenile Belding’s Ground Squirrels provisioned with food were Table 1. Dates of emergence and immergence, body mass (g) at emergence and shortly before
immergence in breeding and non-breeding female European Ground Squirrels (means ± SD; n = 8/10; n.s. = not significant).
Emergence Date Immergence Date Emergence Date Immergence Date
Figure 2.
Plasma progesterone
secretion patterns in
reproductive and non-
reproductive European
Ground Squirrels. Meansin 2-week intervals ± SDare shown (reproductive:n = 7,8,8,8,7,8,8,8,6,1; Seasonal timing and pre-hibernation fattening in breeding and non-breeding European Ground Squirrels fatter than unprovisioned ones, but that these differences disappeared when prehibernation fattening started. Accordingly, our results indicate that prehibernation fattening in this species is a temporally limited process and merely shifted to an earlier period in non-reproductive females. There may be a certain threshold in body fat content that has to be reached to enable hibernation, resulting in an earlier and potential y longer hibernation period in non-breeding females. Early immergence may be beneficial because mortality risk due to predation could exceed that of an extended hibernation period (Michener 1984). Results of previous field studies demonstrated that the frequency of observed predators increased rapidly during the first two weeks of June when the juveniles appeared above ground (Hoffmann et al. unpubl. data).
Although the victims were mainly juveniles, we also observed several kil s of adult individuals.
After weaning, juveniles started to disperse, but the frequency of observed predators only slowly decreased. This could indicate an increased predation risk for adult individuals in the last part of the active season. Apparently, unmated ground squirrels did not use the additional time to get fatter than the others, but preferred to terminate above-ground activity in early summer.
Al females in our enclosures were provided with food, resulting in a good body condition and sufficient fat reserves. Stil , body mass increase rates of al experimental females were within the range of free-living European Ground Squirrels (Millesi et al. 1999b). In non-breeding females, body mass increased very moderately during the first four weeks postemergence. This could be due to a prolonged oestrous period associated with elevated oestrogen secretion (Mil esi et al. 2000). Earlier studies had shown that vaginal oestrus in unmated females, defined by the predominance of cornified cells in vaginal smear samples, lasted for up to two weeks. Oestrus was terminated by late April, corresponding to the end of the mating period in females with access to males. The flexibility to prolong oestrus could be adaptive in cases of low population density or harsh weather conditions in spring (Morton & Sherman 1978; Murie & Harris 1982).
This phenomenon has been found in related species like S. beldingi (Holmes & Landau 1986) and S. tridecemlineatus (Landau & Holmes 1988) as wel as in Woodchucks (Hikim et al. 1991).
As expected, plasma progesterone levels were significantly higher in pregnant females than in non-breeding females during the gestation period in April. Similar results were found in reproductive and non-reproductive Alpine Marmots (Hackländer et al. 2003). Thereafter progesterone secretion increased rapidly in non-breeding females and remained elevated for about four weeks. This could be due to an extended active luteal phase following spontaneous ovulation. Earlier studies have demonstrated a second oestrus cycle after weaning, including spontaneous ovulation and the formation of an active corpus luteum (Mil esi et al. unpubl. data).
Progesterone secretion increased during late lactation and peaked after weaning in June. The extended period of progesterone secretion in non-breeding and postbreeding female European Ground Squirrels seems to be unique among hibernating smal mammals. It may be related to another unusual phenomenon found in this species: females start hibernation about three weeks before males, despite the higher energetic and temporal constraints caused by maternal effort (Mil esi et al. 1999a,b). Elevated progesterone secretion may enhance the progress of prehiberna- tory fattening. Female rats implanted with progesterone, for example, had an increased pro- portion of total body fat compared to control animals (Mendes et al. 1984; Shirling et al. 1981).
Moreover, progesterone could increase food intake rates, as has been shown in rats (Shirling et al. 1981). These effects would be advantageous in animals that have to store body fat in a limited timespan. Future experimental work is needed to investigate these potential effects.
This study clearly demonstrates that the lack of reproductive effort in female ground squirrels led to a shift in seasonal timing, an earlier immergence and probably an extended hibernation Acknowledgements
The study was supported by the Austrian Science Fund (FWF, P13646). We thank Anna Schöbitz
for help in carrying out the endocrine analyses.
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