Recombinant DNA Technique for Spirulina platensis
Yoshikazu KAWATA, NGO Thi Hoai Thu, Seiichi AIBA
National Institute of Advanced Industrial Science and Technology (AIST)
The industrial use of microalgae is important for environmental protection, as it has the potential to assist in the reduction of CO2 emissions, which are now a serious global problem. This should be coupled to wastewater treatments, and be based on mass cultivation and extraction of useful substances from the microalgae. Genetic engineering will be a key to developing appropriate technology; however it has not yet reached the stage of practical application. We have been engaged in. the development of host-vector systems for microalgae. Spirulina platensis is one of the most commercially important species of microalgae. Thus, it is attractive to develop its genetic engineering for practical use, as its stable gene transfer system has not yet been established, due to a lack of suitable vectors and transformation methods. Recently, artificial transposon systems have been extensively developed by extracting essential elements from natural transposons, recently mutated transposase and transposon complex system improved the transformation efficiency by electroporation. We applied another strategy using a natural Tn5 transposon, transposase and cation liposome complex by electroporation to improve transformation efficiency for Spirulina platensis strain C1 (Arthrospira sp. PCC9438). Aggregation of cells became visible after 3 weeks under chloramphenicol selection in 2.0 µg/ml and grew more than 12 months. The transfected chloramphenicol acetyl- transferase (CAT) genes are detected in a genomic DNA area by southern hybridization. Transformed cells showed a CAT activity, but non-transformed cells did not.
We are now engaging to couple wastewater
Transformation transposon complex with cation liposome
treatment and production of chemical materials
IR(Inverted Repeat) Example to transform E coli DH5
by using this genetic modified Spirulina.
Transposon DNA Transposase
“Transformation of Spirulina platensis strain C1
Transposon Cation liposome Cation liposome - transposase complex
transposase-transposon DNA-cation liposome
complex.” Y. Kawata, S.Yano. H.Kojima and T.
Cation liposome
Masaaki. Marine Biotechnology, 6, 355-363, Application to Transformation Efficiency < Transformation Efficiency Spirulina Transformation
Caratteristiche della patologia Informazioni Opistorchiasi Opisthorchis felineus, (O.viverrini, Clonorchis sinensis). Trematode sottile e trasparente di 7 x 1,5 mm, lanceolato, appiattito in senso dorso-ventrale, con una ventosa orale ed una ventrale, ermafrodita. Le uova sono giallo brunastre, opercolate, di 27 x 15 µm, contengono il miracidio già al momento dell’eliminazione.
Recombinant DNA Technique for Spirulina platensis