Reagent kit for the quantitative determination of direct bilirubin in serum. DPD
Quality control
A quality control program is recommended for all clinical laboratories. The analysis of control material in both the normal and abnormal ranges with each assay is recommended Principle
for monitoring the performance of the procedure. Each laboratory should establish Indirect bilirubin is liberated by the detergent. Total bilirubin is coupled with the 3,5- corrective measures to be taken if values fall outside the limits. dichlorophenyl-diazonium-tetrafluoroborate azobilirubin. The absorbance of this dye at 546 nm is directly proportional to the total PERFORMANCES DATA
The following data were obtained using Cobas Mira Plus analyzer Reference value

The test is linear up to 670 µmol/l (39 mg/dl) It is recommended that each laboratory should assign its own normal range. Sensitivity
It is recommended that each laboratory establishes its own range of sensitivity as this is limited by the sensitivity of the spectrophotometer used. Under manual condition the change of 0,001 absorbance is equivalent to 2 µmol/l (0,1 mg/dl) bilirubin concentration Precision
Safety instructions:
Reagent 1:
S26 In case of contact with eyes, rinse immediately with plenty of water and Correlation
Reagent 2:
Comperative studies were done to compare our reagent with another commercial bilirubin S26 In case of contact with eyes, rinse immediately with plenty of water and Linear regression: y=0,975x+3,4 (x=other commercial reagent, y=own reagent) Serum free of haemolysis or EDTA, citrate plasma. Heparin plasma not recommended. Specificity
Bilirubin in serum is light sensitive and it is recommended that serum be stored in the
Ascorbic acid 2,5 mg/dl, hemoglobin 60 mg/dl don’t interfere with the assay up to the Procedure
Avoid direct exposure to light!
Do not use reagents after the expiry date stated on each reagent container label. Do not use products, test solution and reagents described above for any purpose other than descsribed Assay Conditions
For in vitro diagnostic use only!
Pipette into cuvette
Reagent 1
Mix and incubate for 3 minutes, read the absorbance (A1). Calibrator
Reagent 2
Tietz N.W., Clinical guide to laboratory tests, Saunders Co. Thomas L., Clinical Laboratory Diagnostics, TH-Books (1998) Mix and incubate for 5 minutes, read absorbance (A2).∆A=A2-A1 Calibration: (37°C, DPD)
S1:Distilled water
S2: Diagnosticum DunaCal or
Roche C.F.A.S. (Calibrator for automated system)
Calibration is recommended:
- after reagent lot change,
- as required following quality control procedures.
Calculation using calibration
A = Absorbance, C = Concentration

Bilirubin total DPD stable liquid reagent


Microsoft word - curriculum_vitae,_dec_201

1. General Information Surname: Haghdoost Forename: AliAkbar Title: MD-PhD. Academic position: Associate Professor in Kerman University of Medical Sciences Honorary senior Lecturer in London School of Hygiene and Tropical Medicine Sex: Male Date of Birth: 01/09/1971 Place of Birth: Kerman, Iran Marital Status: Married Children: One daughter, One son Iranian Medical Council Registration No: 66136 2

Cenesthopathy in adolescence

Blackwell Science, LtdOxford, UKPCNPsychiatric and Clinical Neurosciences1323-13162003 Blackwell Science Pty Ltd571February 20031075Cenesthopathy in adolescenceH. Watanabe et al. 10.1046/j.1323-1316.2002.01075.x Psychiatry and Clinical Neurosciences (2003), 57 , 23–30 Regular Article Cenesthopathy in adolescence MD, MAMI SUWA, MD AND KAORUKO AKAHORI, MD1 Department of Psychosomatic Medi

© 2010-2017 Pharmacy Pills Pdf