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Microsoft word - ddtechnicalinsert.doc

DRINK DETECTIVE
INTERPRETATION OF RESULTS
(Please refer to the illustration on the test) Drinks Test Device
G TEST - an instant blue colour indicates the presence of illicit GHB in
Technical Insert
a quantity likely to cause a harmful effect. K TEST - an instant red/pink colour indicates the presence of ketamine
Test Strip
in a quantity likely to cause a harmful effect. INTENDED USE
Analytical Specificity
The Drink Detective is a simple to use test developed specially for
B TEST - One or two lines will develop within a minute or so. Their
The following table lists compounds that are positively detected by the detecting all the most common drugs put into drinks to facilitate
Drink Detective Benzo test Strip in water. Detection levels in alcoholic drinks will vary and typical drink spiking doses will be detected. Other drink spiking and drug rape.
benzos will be detected at different sensitivities. NEGATIVE: Two lines appear. This negative result indicates that the
drink is free from benzos or else the benzo level is below the
PRINCIPLE
detectable level (500 ng/mL). The line on the right side is a ‘control Concentration
Concentration
Compound
Compound
The G test detects particular changes in most drinks caused by spiking line’ which confirms sufficient specimen volume, adequate membrane with clandestine GHB. A drink adulterated with illicitly manufactured wicking and correct procedural technique. GHB will instantly turn the G paper blue; the paper with most drinks will remain pink. Only a few drinks, like those which are oily or milk like and *NOTE: The shade of red of the left hand test line may vary, but it
have the same property as illicit GHB and will give a false positive should be considered negative whenever there is even a faint pink line. POSITIVE: One red line appears No line appears in the test region
Ketamine is a chemical known as a secondary amine. It can be (T). This positive result indicates that the benzo concentration exceeds detected with a special reagent in the K test, with little cross reactivity to other substances in drinks. These drugs produce a red/pink colour change. If no drug is present the paper will usually turn a white or grey colour. This test has proved to be very sensitive, but since the positive INVALID: No lines appear. Insufficient specimen volume or incorrect
reaction is a red/pink colour, take care not to confuse a slight red/pink procedural techniques are the most likely reasons for test failure. tint from the natural colour in red wine and orange juice. Positive Review the procedure and repeat the test using a new test. If the Cross-Reactivity
problem persists, contact your local distributor. The following compounds show no cross-reactivity when tested with LIMITATIONS
the Benzo Test Strip (Urine) at a concentration of 100 µg/mL. Benzodiazepines, or benzos, are a group of widely used drugs, and so are freely available. Flunitrazepam (Rohypnol®) is a sleeping pill also The Drink Detective is a presumptive test intended to screen out all drinks which contain the common substances used in drug Non Cross-Reacting Compounds
used as a sedative and a pre-anaesthetic drug. It has the effect of rape. A secondary analytical method must be used to obtain a causing drowsiness and amnesia. Chlordiazepoxide (Librium) and confirmed result. Gas chromatography/mass spectrometry diazepam (Valium) are common benzos. The B strip is an (GC/MS) is the preferred confirmatory method. N-Acetylprocainamide Ethyl-p-aminobenzoate immunoassay, and uses antibodies, instead of chemicals, to detect It is possible that technical or procedural errors, as well as other drugs. The benzo in a drink will interact with the test strip to produce interfering substances in the drink may cause erroneous results. lines on the test area. One line means the drug is present (positive), A positive result indicates presence of a drug but does not two lines means it is absent (negative). The antibodies are affected by high concentrations of alcohol and so may not work with some neat A negative result may be obtained when drug is present but spirits, but in this case no lines at all will form. However this type of The Drink Detective gives a qualitative, presumptive analytical
STORAGE AND STABILITY
test result. If you wish to take legal action on the results a more
specific alternate chemical method must be used in order to
The kit can be stored at room temperature or refrigerated (2-30°C). The obtain a confirmed analytical result. Gas chromatography/mass
Drink Detective should remain in the sealed pouch until use. DO NOT
spectrometry (GC/MS) is the preferred confirmatory method.
FREEZE. Do not use beyond the expiry date.
Professional judgment should be applied to any positive result.
Consider seeking help from the Drink Spiking Hotline described
DIRECTIONS FOR USE
on the product pack.
Bring the pouch to room temperature before opening it. Remove the Drink Detective from the sealed pouch and use it as soon as PERFORMANCE CHARACTERISTICS OF THE B TEST
(±) - Brompheniramine Ketoprofen Quinidine Remove the dropper and use it to sample the drink. The benzo test was compared with another brand leader using spiked Put one drop on each of the G Test and K test and observe the immediate colour reaction. Do not rely on the colours later than 30 seconds after you have tested the drink. Total Results
Put four drops onto the end of the B Test strip, and observe % Agreement with
the magenta colour running along the strip. this Rapid Test Kit
When compared at 500 ng/mL cut-off with GC/MS, the following results were to another. The G paper and the K paper also detect a range of other DRINKS THAT HAVE BEEN TESTED
The following drinks give a negative reaction with the G, K and B test but the Drink Detective identifies the presence of the following levels of drug. Ecgonine methylester Papaverine Tyramine [1R,2S](-) Ephedrine Pentazocine-hydrochloride Verapamil MANUFACTURER OF THE DRINK DETECTIVE
The product is manufactured under license by SureScreen Diagnostics Ltd for Bloomsbury Innovations Ltd, 5E Bloomsbury Street, London, PERFORMANCE CHARACTERISTICS OF THE G TEST
WC1B 3QE. Tel: +44 (0)207 6310707 Fax: +44 (0)207 6310704
It is very important to note that the particular effects on individuals is Email: sales@bloomsburyinnovations.com
highly variable for GHB. Consumption of other substances in Web: www.drinkdetective.com
combination with GHB will also greatly influence the effects. 200 Safe Drinking Practices. - ways to stay alert and be on your guard.
The dosages and side effects commonly reported in the literature are illustrated below. Interconversion of parts per million (ppm) - grams - • Tell someone where you go & what time you’re expected home. teaspoons per standard 250mL beverage has been included for • Avoid drinking alone. Friends should watch-out for each other. Effect Zero Mild Standard Strong Heavy Overdose Hypnosis Coma Death • Appoint a nominated drink watcher (e.g. the non-drinking driver?). • Plan your night out, arranging your journey to and from home. • Remember the biggest drug rape drug is alcohol. It affects your actions / reactions as well as ability to be alert. Calculated for a standard 250mL beverage. General onset of effect is 5-20mins. Duration 1-5h. • Stay aware of what is going on around you and keep away from situations you do not feel comfortable with. Know your alcohol limit. The G test has been formulated to detect a positive drink when it contains • Never accept a drink from anyone you do not completely trust. 1000 mg (1 gram) of illicit GHB per 250 ml of drink. Sensitivities will vary from one drink to another. The G test is not formulated to react with • Don’t share or exchange drinks. Don’t drink discarded drinks laboratory standards or controls, only proper illicit GHB. • Don't leave your drink unattended, even when going to the toilet. PERFORMANCE CHARACTERISTICS OF THE K TEST
• Drugs can be put in soft drinks, tea, coffee, etc., as well as alcohol. It is very important to note that the particular effects on individuals is • Drinking from a bottle and keeping your thumb over the top is a good highly variable for ketamine. Consumption of other substances in idea. Just remember that if you leave it unattended you may not be combination with ketamine will also greatly influence the effects. able to see if anything has been put in it. • If you return to your drink and it has been moved, looks different, The dosages and side effects commonly reported in literature and the appears to have been topped-up or tastes strange, don't take a chance. media are illustrated below. Interconversion of parts per million (ppm) Test it with the Drink Detective. Test your favourite drinks, and be and milligrams per standard 250mL beverage has been included for • Consider very carefully whether you should leave the pub, club or • If you begin to feel really drunk after only a drink or two, seek help from a trusted friend or a member of the club or pub management. • Remember this crime happens to men as well as women. Calculated for a standard 250mL beverage. General onset of effect is 5-20mins. Duration 4-8h. The K test has been formulated to detect a positive drink between 100and 200 mg per 250 ml drink. Sensitivities will vary from one drink

Source: http://dev-www.drinkdetective.ca/science/DDtechnicalinsert.pdf

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Point of View Metabolic or Pseudometabolic Syndrome? Luis Cláudio Lemos Correia1, Adriana L. Latado2, José Augusto Barreto-Filho3 Escola Bahiana de Medicina1; Universidade Federal da Bahia2, Salvador/BA; Universidade Federal de Sergipe3, Aracaju, SE, Brazil A predictive model is created based on the following: (1) Metabolic syndrome has been proposed as a predictor of identification of v

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