Durante mucho tiempo no había principios uniformes para la Atribución de nombres a los antibióticos https://antibioticos-wiki.es . Más a menudo se les llama por el nombre genérico o especie del producto, con menos frecuencia-de acuerdo con la estructura química. Algunos antibióticos se nombran de acuerdo con el lugar donde se asignó el producto.
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Anti α-Gal Immune Response Following Porcine
Bioprosthesis Implantation in ChildrenChun Soo Park1, Seong-Sik Park2, Sun Young Choi2, Sun Hee Yoon1, Woong-Han Kim1, Yong Jin Kim1,2
1Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine, 2Seoul NationalUniversity Hospital Clinical Research Institute, Xenotransplantation Research Center, Seoul, Republic of KoreaBackground and aim of the study: Porcine bioprosthe- tively. All serum samples were analyzed using an ses have been widely used in cardiac surgery in the enzyme-linked immunosorbent assay to investigate treatment of valvular heart disease. However, in the α-Gal immune response. younger patients, their use has been limited by early Results: There were no operative deaths or complica- failures known to be associated with an immune tions. There was no statistically significant differ- response and subsequent degeneration. The natural ence between the titers of anti α-Gal antibodies of antibodies directed at Galα1, 3-Galβ1-4GlcNAc-R(α- the PVR and re-PVR groups. The titer of anti α-Gal Gal), have been thought to initiate an immune antibodies (IgM and IgG) was decreased on the first response in humans transplanted with porcine organ postoperative day, but increased in the first postop- xenografts. The study aim was to determine the anti erative week, regardless of the isotype. Whilst the
α-Gal immune response following commercial titer of the anti α-Gal IgM antibody began to porcine bioprosthesis implantation in children. decrease after three weeks postoperatively, the titer Methods: Between January 2008 and April 2008, 19 of anti α-Gal IgG antibody remained increased after consecutive patients underwent pulmonary valve two months. replacement (PVR) with a commercially available Conclusion: The implantation of a porcine biopros- porcine bioprosthesis for an incompetent pulmonary thesis elicits the increased formation of anti α-Gal valve with congenital heart diseases. The median age antibodies during the early postoperative period in at surgery was 132 months (range: 14-330 months). children, with different patterns between the two Previous PVR with a porcine bioprosthesis had been isotypes. The IgM antibody response was rapid and performed in seven patients at a median of 44 transient, while the IgG antibody response was months (range: 26-117 months) before surgery (re- longer and more delayed. PVR group). Sera were obtained sequentially five times: immediately before surgery, and at one day, one week, three weeks, and two months postopera-
The Journal of Heart Valve Disease 2010;19:124-130
Xenoreactive natural antibodies directed at the
designated to the α-Gal epitope in humans, and
Galα1, 3-Galβ1-4GlcNAc-R (α-Gal), epitope are known
although anti α-Gal antibodies are produced through-
to be a major barrier in xenotransplantation. The natu-
out the patient’s lifetime due to exposure to the gut
ral anti-pig antibodies in human serum react predomi-
flora (3), the titers of natural antibodies show individ-
ual differences (4). In xenotransplantation, anti α-Gal
α-Gal-xenoreactive antibodies comprise at least 80-
antibodies mediate the hyperacute rejection with com-
90% of anti-pig antibodies in humans (2).
plement activation, and delayed or chronic rejection
Approximately 1% of the circulating antibodies are
with an antibody-dependent cellular cytotoxicitymechanism (3,5-7).
The α-Gal epitope is expressed on a variety of tissues
in pigs, including the cardiac valve endothelium (1,8-
Yong Jin Kim MD, PhD, Department of Thoracic and Cardiovascular
10); recently, the presence of the α-Gal epitope was
Surgery, Seoul National University College of Medicine, SeoulNational University Hospital Clinical Research Institute,
documented in commercial bioprosthetic valves treat-
Xenotransplantation Research Center, 28 Yongon-dong, Jongno-gu,
Many patients who undergo procedures on the right
Kim 3845 r1.qxd:Layout 1 25/1/10 10:02 Page 125
Anti α-Gal immune response 125
Table I: Patient preoperative characteristics.
*Diagnosis: AORPA: Anomalous origin of right pulmonary artery; cc-TGA: Congenitally corrected TGA; PA: Pulmonaryatresia; PS: Pulmonary stenosis; PVR: Pulmonary valve replacement; TGA: Transposition of the great arteries; TOF: Tetralogy of Fallot; VSD: Ventricular septal defect. +Preoperative medication: ADT: Aldactone; DCZ: Diclozide; DGX: Digoxin; ENL: Enalapril. ‡Valve: CE: Carpentier-Edwards Bioprosthetic Valved Conduit Model 4300 (Edwards Lifesciences); H: Hancock® II Aortic,Medtronic™; SJM: Epic™ SUPRA valve Aortic, St. Jude Medical, Inc.
ventricular outflow tract require pulmonary valve
increased formation of anti α-Gal antibodies; and (ii)
replacement (PVR), with or without a conduit.
monitor perioperative changes in the titer of the anti α-
Although, in most centers (including that of the pres-
Gal antibodies (IgM and IgG) over time.
ent authors) the prosthesis of choice for PVR is ofporcine origin, such bioprostheses have a limited dura-
Clinical material and methods
bility due to valve degeneration that occurs more fre-quently in younger patients (8,9,12-14). The
Patients
degeneration of bioprostheses is multifactorial, and
Between January 2008 and April 2008, 19 consecutive
includes immunologic reactions, foreign body reac-
patients underwent PVR with porcine bioprostheses
tions, blood-surface interactions, chemical factors,
produced by three manufacturers: the EpicTM SUPRA
infection, mechanical factors, material fatigue, and sur-
valve aortic (St. Jude Medical, Inc.; the HANCOCK® II
gical factors (15). Although the mechanism leading to
aortic (Medtronic); and the Carpentier-Edwards
premature degeneration of the implanted bioprosthe-
Bioprosthetic valved conduit Model 4300 (Edwards
sis is not yet fully understood, the immune response
Lifesciences). The patients’ diagnoses were tetralogy of
has been considered to play an important role as an
Fallot or variants thereof in 12 cases, truncus arteriosus
initial trigger of the degeneration process (16), and α-
in three, congenitally corrected transposition of the
Gal has been noted as the major epitope.
great arteries with pulmonary stenosis in two com-
The aim of the present study was to: (i) investigate
plete transposition of the great arteries with pul-
whether bioprosthesis implantation elicits an
monary stenosis in one, and anomalous origin or right
Kim 3845 r1.qxd:Layout 1 25/1/10 10:02 Page 126
126 Anti α-Gal immune responseFigure 1: Changes in anti α-Gal activity in patient #14(PVR group) and patient #17 (re-PVR group). PVR:Pulmonary valve replacement; OD: Optical density; Figure 2: Changes in the anti α-Gal antibody titer in theindividual groups. A) Re-PVR IgM titer. B) Re-PVR IgGtiter. C) PVR IgM titer. D) PVR IgG titer. PVR:
pulmonary artery from the ascending aorta each in one
Pulmonary valve replacement; POD: Postoperative day.
case. A previous PVR with a porcine bioprosthesis hadbeen performed in seven patients (the re-PVR group)at a median of 44 months (range: 26 to 117 months)
before surgery, while the remaining 12 patients under-
An ELISA analysis was used to determine the
went PVR for the first time (the PVR group). The pre-
activity of IgM and IgG isotypes of the anti α-Gal anti-
operative characteristics of the patients are listed in
bodies. Bovine serum albumin (BSA) containing syn-
Table I. The median age at surgery was 132 months
thesized α-Gal (α-Gal-BSA), which was prepared
by conjugation of α-Gal linker type 1 (Genkem,
Postoperatively, all patients followed a similar med-
Seoul, Korea) and bovine serum albumin (Armresco,
ication protocol. Serum samples were obtained five
Solon, OH, USA) with 1-ethyl-3-[3-dimethylamino-
times from each patient: immediately before surgery,
propyl]carbodiimide hydrochloride (Pierce, USA) in
at postoperative day 1, during the first and third post-
conjugation buffer (MES buffered saline pack; Pierce),
operative weeks, and during the second postoperative
was used as a solid-phase antigen. Microtiter plates
month. All sera were stored in EDTA tubes and ana-
were coated with 100 μl per well of α-Gal-BSA in PBS
lyzed within one day of being obtained.
buffer (pH 7.4) (at a concentration of 1 μg/ml for the
The study was approved by the institutional review
IgM isotype, or 2.5 μg/ml for the IgG isotype), and
board/ethical committee of the authors’ institution,
incubated for 1 h at 37°C. The plates were then washed
and informed consent was obtained from each of the
with deionized water. Aliquots of the patient’s serum
patients. The study was conducted in accordance with
(100 μl per well) were added to the α-Gal-BSA-immo-
bilized wells at a serial two-fold dilution, from 1:40 to1: 2,560 in BSA-Triton X-100 (pH 7.4, PBS, 3% BSA,
Operative technique
0.01% Triton X-100). The plates were then incubated
In all patients, PVR was performed using moderate
for 1 h at 37°C. Donkey anti-human IgG and IgM anti-
hypothermic cardiopulmonary bypass (CPB) and left
bodies (Jackson, Human Research Laboratories Inc.)
atrial venting through the right upper pulmonary vein,
were used as a secondary antibody at a dilution of
with or without myocardial ischemia, under ventricu-
1:15,000 for IgM and 1:10,000 for IgG in BSA-Triton X-
lar fibrillation for isolated PVR (n = 10) and after
100. The optical density (OD) was measured at 450 nm
release of the aortic clamp in patients with additional
using the Thermo Electron-Lab Systems (Multiskan
Kim 3845 r1.qxd:Layout 1 25/1/10 10:02 Page 127
Anti α-Gal immune response 127
Table II: Anti α-Gal titers in the two groups.*
Values are mean ± SD. *Titer defined as the dilution at 1.0 of the OD. PVR: Pulmonary valve replacement; POD: Postoperative day. Statistical analysis
immediately postoperatively (at one day) and then
Background OD-values were subtracted from the
increased after one week, when compared to the pre-
test OD-values. The titer was defined as the dilution at
operative OD, regardless of the isotype. The ODs of the
1.0 of the OD. All data were presented as mean ± SD,
IgG isotype remained elevated at the end of the study
or median plus range, using Microsoft Excel 2007.
(at 60 days after surgery), but those of the IgM isotype
Comparisons were performed using either Student’s t-
began to decrease at three weeks postoperatively.
test or a paired t-test. A p-value <0.05 was considered
Although the postoperative titer seemed to be high-
er in the re-PVR group (Table II; Fig. 2), there was nosignificant difference between the two groups (PVR
and re-PVR) with regards to the anti α-Gal IgM andIgG titers (Table II). The change in titer of the anti α-
Clinical results
Gal antibodies with time was similar in each patient
No early deaths occurred among the patients. The
(see Fig. 1). The immediate postoperative (one-day)
median CPB time was 167 min (range: 94 to 392 min),
titer appeared to decrease, though not statistically sig-
while in nine patients aortic cross-clamping was
nificantly (Table III; Fig. 3). At one week after surgery
required for additional intracardiac procedures. The
the titer increased significantly, up to seven-fold in
median myocardial ischemic time was 108 min (range:
IgM and 32-fold in IgG (Table III; Fig. 3). The IgM titer
55 to 140 min). In all patients, the preoperative prepa-
began to decrease at three weeks after surgery, but that
ration, anesthetic management and operative strategy
of IgG increased from three weeks and was maintained
were similar and the hospital course was uneventful,
until the end of the study (at two months after surgery)
and all were discharged within 10 days after surgery,
without complications. Treatment with an angiotensin-converting enzyme inhibitor was required during the
Discussion
early postoperative period in eight patients, and anti-coagulation for six months in all patients. The median
As α-Gal is a major barrier for xenotransplantation,
duration of follow up was 11 months (range: 8 to 14
much effort has been expended to avoid the immune
months). All patients were in NYHA functional class 1,
response directed against it, including immunoglobu-
and none of the patients was administered any cardiac
lin or enzymatic treatment and genetic manipulation
medication (except warfarin) at the last follow up
(α1,3-galactosyltransferase knock-out) (17-19).
Unfortunately, the α1,3- galactosyltransferase knock-out pig is not yet available within a clinical setting, and
ELISA: Anti α-Gal antibody titer
other methods such as enzymatic removal of the α-Gal
Results from random samples (patient #14 of the
epitope have caused only a delay in the rejection. The
PVR group and patient #17 of the re-PVR group) are
major antibodies that are thought to initiate the hyper-
shown in Figure 1. In all cases, the OD decreased
acute rejection of porcine xeno-organs include IgM
Kim 3845 r1.qxd:Layout 1 25/1/10 10:02 Page 128
128 Anti α-Gal immune responseTable III: Anti α-Gal titer in all patients (versusFigure 3: Changes in the anti α-Gal antibody titer in allpatients. A) Changes in IgM titer. B) Changes in IgG titer.
turnover would not be expected) these patients still
*Titer defined as the dilution at 1.0 of the OD. PVR: Pulmonary valve replacement;
develop calcification soon after transplantation along
with valve destruction. This suggests that anotherprocess is involved, which may be an immune reaction.
xenoantibodies; however, IgG xenoantibodies can also
Manji et al. (24) reported that significant inflamma-
mediate complement activation as well as antibody-
tion of the xenografts, as well as a significant humoral
dependent cellular cytotoxicity mechanisms (6).
response to the xenografts, had occurred within a short
In cardiac surgery, non-viable porcine bioprosthetic
period of time, and that the calcification correlated
valve implantation has been performed for four
with the amount of inflammation in a young animal
decades, and today the main concern of cardiac sur-
model. Konakci and colleagues (16) noted that degen-
geons following the implantation of a bioprosthesis is
eration of the bioprosthetic valve begins with the pen-
not a catastrophic hyperacute rejection but rather a late
etration of immunoglobulins into the valve-matrix,
degeneration of the implanted valve, which often
macrophages onto the valve surface; the process is
Although the currently available porcine biopros-
then completed with collagen breakdown and calcifi-
thetic valves include a variety of design, manufactur-
cation. The same group (16) reported that the α-Gal
ing, and treatment options, the vast majority are
epitope still existed on the commercial porcine bio-
glutaraldehyde-treated. It is generally accepted that
prosthetic valve, thus confirming the findings of
bioprosthetic valve degeneration is due to calcification
Kasimir et al. (11), and that the bioprosthesis implanta-
of the valve and tissue disruption or tearing.
tion elicited a specific humorally-mediated immune
Glutaraldehyde fixation has been introduced to reduce
response directed against α-Gal. Several in vivo stud-
valve antigenicity and to improve its mechanical
ies have reported that the titer of anti α-Gal IgM or IgG
strength (20). However, the failure of glutaraldehyde-
antibodies was increased, as measured using ELISA
treated porcine xenografts in clinical series has been
reported, while others have shown that glutaralde-
In the present study, it was observed that the titer of
hyde treatment does not remove xenograft antigenici-
anti α-Gal antibodies changed with different patterns
ty. Thus, one of the major causes of bioprosthesis
between the two isotypes. At one week after surgery,
degeneration is an immune reaction (21-24).
the anti α-Gal antibody titer increased, regardless of
The immune mechanism has been considered to play
the isotype. Whilst the anti α-Gal IgM antibody titer
an important role as an initial trigger of the degenera-
began to decrease after three weeks, that of IgG was
tion process, and the α-Gal epitope is the major antigen.
maintained over two months after surgery. The imme-
In the same context, the younger the patient’s age, the
diate postoperative (day 1) titers showed a tendency to
more frequently will valve degeneration occur, which
decrease, which may be ascribed to the binding of cir-
suggests a more active and robust immunologic
culating anti α-Gal antibody to the α-Gal isotope on
response to xenoantigens (8,9,14). Simionescu (25) noted
the valve and/or a dilutional effect of CPB or intravas-
that excessive wear and tear, and a higher calcium
turnover secondary to growth, is responsible for early
There was no statistically significant difference in the
calcification in children; however, even after the adoles-
anti α-Gal antibody titer between the PVR and re-PVR
cent period (young adulthood, when a high calcium
groups. Before surgery, the anti α-Gal antibody titer
Kim 3845 r1.qxd:Layout 1 25/1/10 10:02 Page 129
Anti α-Gal immune response 129
was similar between the two groups, which suggested
that the antigenicity of the α-Gal epitope on a previ-
4. Buonomano R, Tinguely C, Rieben R, Mohacsi PJ,
ously implanted valve may have decreased and almost
Nydegger UE. Quantitation and characterization of
disappeared with time. However, the mean postopera-
anti-Galα1-3Gal antibodies in sera of 200 healthy
tive titer (on days 21 and 60) seemed to be higher in the
persons. Xenotransplantation 1999;5:173-180
re-PVR group (though not statistically significantly),
5. Galili U, LaTemple DC, Walgenbach AW, Stone KR.
such that the possibility of an influence due to previ-
Porcine and bovine cartilage transplants in
ous exposure on the immune response to the second
cynomolgus monkey. Transplantation 1997;63:646-
exposure, perhaps by host sensitization, may not be
6. Cooke SP, Pearson JD, Savage COS. Human IgG
xenoreactive antibodies mediate damage to porcine
Study limitations
endothelial cells in vitro by both humoral and cel-
The primary limitation of the study was the small
lular mechanisms. Transpl Immunol 1997;5:39-44
patient numbers, and the wide range of age and body
7. Lin SS, Kooyman DL, Daniels LJ, et al. The role of
weight. The study was also limited by the use of dif-
natural anti-Galα1-3Gal antibodies in hyperacute
ferent types of bioprosthesis with different anticalcifi-
rejection of pig-to-baboon cardiac xenotransplants.
cation treatments. Despite these limitations, the
implantation of a porcine bioprosthesis was found to
elicit the anti α-Gal immune response in each patient
McKenzie IFC. Studies on human naturally occur-
ring antibodies to pig xenografts. Transplant Proc1993;25:2917-2918
In conclusion, porcine bioprosthesis implantation
9. Stone KR, Walgenbach AW, Abrams JT, Nelson J,
elicits an increased production of anti α-Gal antibodies
Gillett N, Galili U. Porcine and bovine cartilage
in humans, albeit with different patterns between the
transplants in cynomolgus monkey: I. A model for
two isotypes (IgM and IgG). Notably, the IgM antibody
chronic xenograft rejection. Transplantation
response was more rapid and transient, while that of
IgG was longer and more delayed. The most important
10. Farivar RS, Filsoufi F, Adams DH. Mechanisms of
requisites for the ideal valve substitute in cardiac sur-
Gal(alpha)1-3Gal(beta)1-4GlcNAc-R (alphaGal)
gery are reliability and durability. Although an
expression on porcine valve endothelial cells. J
immunologic response to the xenoantigen other than
α-Gal (non-gal antigen) has been reported (27-29), the
11. Kasimir MT, Rieder E, Seebacher G, Wolner E,
α-Gal epitope is a major antigen that must be over-
Weigel G, Simon P. Presence and elimination of the
come in pig-to-human transplantation, including bio-
xenoantigen gal (alpha1,3) gal in tissue-engineered
prosthesis implantation. For this reason, further
heart valves. Tissue Eng 2005;11:1274-1280
studies are required to either avoid or minimize the
12. Silver MM, Pollock J, Silver MD, Williams WG,
immune response to the α-Gal epitope, including
Trusler GA. Calcification in porcine xenograft
decellularization as well as enzymatic or genetic
valves in children. Am J Cardiol 1980;45:685-689
13. Jamieson WRE, Rosado LJ, Munro AI, et al.
Carpentier-Edwards standard porcine bioprosthe-
Acknowledgements
sis: Primary tissue failure (structural valve deterio-
These studies were supported by grants from the
Korea Health 21 Research & Development Project,
Korean Ministry of Health, Welfare & Family (A04004-
14. Fann JI, Miller DC, Moore KA, et al. Twenty-year
clinical experience with porcine bioprostheses. AnnThorac Surg 1996;62:1301-1312
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TRATAMIENTO DE LA HIPERTENSIÓN PULMONAR PEDIÁTRICA CON SUSPENSIÓN ORAL DE 2,5 MG/ML DE SILDENAFILO. INTRODUCCIÓN La hipertensión arterial pulmonar constituye uno de los tipos más comunes de hipertensión pulmonar, pudiendo ser de naturaleza idiopática o secundaria a otras etiologías. Su sintomatología más común es lo que se denomina disnea de esfuerzo, es decir, la dificultad al resp
"LOS DERECHOS ECONÓMICOS SOCIALES Y CULTURALES EN UN MUNDO GLOBALIZADO DR. ALIRIO URIBE MUÑOZ CORPORACIÓN COLECTIVO DE ABOGADOS - COLOMBIA 1- Base constitucional colombiana para la estrategia de exigibilidad 2- Acciones judiciales en el plano nacional que nos sirven para exigir los derechos económicos, sociales y culturales - Experiencias y expectativas 2.1- La acción de