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Microsoft word - course outline of phrm-409 _advanced pharmaceutical analysis_ fall 2012

Course Information and Outline
PHRM 409: Advanced Pharmaceutical Analysis [Credit: 4]
Fall, 2012
Department of Pharmacy

Instructor: Dr. Chowdhury Faiz Hossain,

Professor and Dean, FSE.
Section 2
Class Hours: 12:40 1:40
Course Objective:

 To give some ideas on advanced level about the principles and applications of NMR
spectroscopy and Mass spectrometry in order to understand how to characterize the structure of an unknown compound. And combined application of all spectroscopic methods.  To enable the students understand the principles of and applications of HPLC in advanced  To enable the students acquainted with some advanced analytical techniques for pharmaceutical products like atomic absorption spectroscopy and radioimmunoassay DESCRIPTION OF THE TOPICS:
1. High Performance Liquid Chromatography (HPLC)
Objectives of using HPLC techniques, principle, instrumentation, different types of column used in HPLC techniques with their uses, chemistry stationary and mobile phases and their retention mechanism, application of HPLC in pharmaceutical analysis. 2. 1H- and 13C-Nuclear Magnetic Resonance Spectroscopy
Introduction, theory and principle of NMR, Instrumentation, chemical shift of NMR, spin-spin interactions, interpretation of a NMR spectrum, relative peak area, multiplicity of the signal and coupling constant, applications of NMR spectroscopy in pharmaceutical analysis. 3. Mass Spectrometry
Introduction, basic principle/theory, instrumentation, mass spectrum, molecular ion or parent ions, mass fragmentation, determination of molecular formula. 4. Combined applications of UV, IR, NMR and MS for structural elucidation
Practice of the determination of the structures of unknown compounds by utilizing the data gained from UV, IR, NMR and MS. 5. Atomic absorption spectroscopy
Basic concepts of atomic absorption spectroscopy, its distinction from other spectroscopic techniques, merits and demerits, instrumentation, important aspects of AAS and its application in pharmaceutical analysis like assay of total zinc in Insulin Zinc suspension. 6. Radioimmunoassay
Introduction, theory, instrumentation, methodology of the assay, applications of radioimmunoassay (RIA) in pharmaceutical analysis like radioimmunoassay of morphine, flurazepam, barbiturates, etc. Lab class:
1. Determination of the potency of acetaminophen from paracetamol tablet dosage form by UV 2. Preparation of standard curve (absorbance versus concentration) of paracetamol at different 3. Determination of the potency of cetirizine from cetirizine HCl preparation by UV 4. Identification of the desired component by Thin-layer chromatographic (TLC) analysis of Evaluation :
Students will be evaluated through the events as follows:
Grading Scheme
Class participation /Attendance 05% Class tests/quiz (announced / unannounced) 10% Lab (practical +oral)

1. Introduction to Spectroscopy By Donald L. Pavia, Gary M. Lampman, George S. Kriz 2. HPLC for Pharmaceutical Scientists by Yuri Kazakevich and Rosario Lobrutto 3. Practical High-Performance Liquid Chromatography by Veronika R. Meyer 4. Analytical Instrumentation By Gillian Mcmahon 5. HandBook of Pharmaceutical Analysis by HPLC by Ahuza and Dong 6. Immunoassay A Practical Guide – By Brian Law 7. Pharmaceutical Analysis - By David G. Watson 8. Lecture and Lecture materials (slides)
Special Instructions:

Attendance Policy: 20% or more absences of the class session for the semester constitutes
unsatisfactory attendance and will place in jeopardy of being dropped from the course with the Final
Grade of ‘F’.
Quizzes and tests: Written Quizzes and tests will be given regularly with or without prior notice
throughout the semester.



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