TECHNICAL SHEET Rev. 0 of 21.10.2008 Pag. 1 of 2 MUELLER HINTON II MOD. AGAR Medium for susceptibility test (Kirby-Bauer method). TYPICAL FORMULA (g/L) DESCRIPTION MUELLER HINTON II MOD. AGAR is used for antimicrobial susceptibility testing of rapidly growing aerobic microorganisms by the disk diffusion technique. MUELLER HINTON II MOD. AGAR is a medium recommended by NCCLS and contains controlled levels of calcium and magnesium ions in order to obtain aminoglycosides inhibition zones against Pseudomonas aeruginosa and tetracycline inhibition zones against staphylococci within the ranges suggestad by NCCLS M100-S12: Mg ++=20-25 mg/L, Ca++=50-100 mg/L. PRINCIPLE Casamino acids and meat extract are a source of amino acids, nitrogen, minerals, vitamins, carbon and other factors which increase the growth of microorganisms. Starch acts as a protective substance against toxic molecules which can be present in the medium. Hydrolysis of starch during sterilization supplies a little amount of glucose which represents a source of energy. Agar is the solidifying agent. Kirby-Bauer method is based on the diffusion, through the agar, of antimicrobial substances which soaks paper disks: microrganism growth shows an inhibition halo around the disk and the diameter of the halo is correlated to the Minimal Inhibiting Concentration (MIC). TECHNIQUE Transfer 4-5 colonies in an appropriate broth. Place it in a 37°C incubator until an opacity is obtained equivalent to the standard opacity of 0.5 on the MacFarland scale. Introduce a sterile swab into the inoculum and inoculate the agar passing 2 or 3 times onto the entire surface. Press the disk containing the antimicrobial on the agar surface. Incubate at 36+/-1°C for 18 hours, measure the inhibition zone with a compass and compare to the NCCLS recommended zone ranges. INTERPRETATION OF RESULTS Compare obtained values of inhibition halo diameter with the values reported on NCCLS M100(M2) document. STORAGE 10-25°C away from light, until the expiry date on the label or until signs of deterioration or contamination are evident. WARNING and PRECAUTIONS The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. The product is designed for In vitro diagnostic use and must be used only by properly trained operators. DISPOSAL of WASTE Disposal of waste must be carried out according to national and local regulations in force. REFERENCES
Bauer et al. (1966). J. Clin. Pathol. 45:493-496.
Mueller, J.H., and Hinton. 1941. Proc. Soc. Exp. Biol. Med. 48: 330-333.
3. NCCLS. Performance standards for susceptibility testing; Twelve Informational Supplement. NCCLS Document M100-S12, January
Liofilchem s.r.l. Via Scozia-Zona industriale - 64026 Roseto degli Abruzzi Tel. +39.085.8930745 - Fax +39.085.8930330 Web site:filchem@liofilchem.net TECHNICAL SHEET Rev. 0 of 21.10.2008 Pag. 2 of 2 PRODUCT SPECIFICATIONS NAME MUELLER HINTON II MOD. AGAR PRESENTATION Ready plates (90 mm) containing 22+/-1 ml of medium. STORAGE 10-25°C PACKAGING pH OF THE MEDIUM 7.4 ± 0.1 USE MUELLER HINTON II MOD. AGAR is used for antimicrobial susceptibility testing of rapidly growing aerobic microorganisms by the disk diffusion technique. MUELLER HINTON II MOD. AGAR is a medium recommended by NCCLS. TECHNIQUE Refer to technical sheet of the product. APPEARANCE OF THE MEDIUM Amber medium, slightly opalescent. SHELFLIFE 6 months QUALITY CONTROL 1. Control of general characteristics, label and print
Sterility control7 days at 25 ± 1°C, in aerobiosis7 days at 36 ± 1°C, in aerobiosis
Microbiological controlIncubation conditions:18-24 h at 36 ± 1°C
TABLE of SYMBOLS Liofilchem s.r.l. Via Scozia-Zona industriale - 64026 Roseto degli Abruzzi Tel. +39.085.8930745 - Fax +39.085.8930330 Web site:filchem@liofilchem.net
Policy for Assessment and Recognition of Overseas Assessment and Regulatory Authorities Preamble The Osteopathy Board of Australia (OBA) wishes to offer a Competent Authority Pathway (CAP) for registration of osteopaths who have qualifications gained outside Australia and New Zealand. Overseas assessing authorities may offer, or have offered, more than one pathway to registration.
C5: Methodological and Philosophical Issues in PhysicsThis paper evaluates how the symmetry problem that has recently beenraised [2] for Friedmann-Lemaˆıtre-Robertson-Walker spacetimes in generalrelativity (GR) and according to which spacetime structuralism seems com-mitted to the claim that the universe has no spatial extension—it consistsof one lonely point—is transposed into the causal s
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