VON KOSSA method for calcium 04 – 170801 CND Code: W01030799 Expected aim
Product for the preparation of cyto-histological samples for optical microscopy.
Application
To demonstrate deposits of calcium ions in tissue sections.
Principle
This method is based on a substitution reaction. Tissue is treated with a silver nitrate solution; cationic silver replaces calcium in the original salt thus forming a silver salt which can be showed by a reduction to metallic silver. Reaction is as follows: CaCO3 + AgNO3 = Ag2CO3 + Ca(NO3)2 Ag2CO3 + 2H + = 2Ag + H2O + CO2 Calcium salts liable to react with silver nitrate are: carbonate, phosphate, oxalate, sulfate, urate, chloride, sulfocyanide. Since calcium is present in mammalian tissue essentially in the form of calcium carbonate and calcium phosphate, this method is suitable to point it out. To prevent false results caused by the presence of uric acid and urates in the tissue, these salts have been solubilized in a lithium carbonate saturated solution.
Put on the section 10 drops of reagent A : leave to act 10 minutes.
Put on the section 10 drops of reagent B: leave to act in the dark 1 hour.
Put on the section 5 drops of distilled water and add 10 drops of reagent C: leave to act 5 minutes (Until silver salts become black).
Put on the section 10 drops of reagent D: leave to act 5 minutes.
10) Put on the section 10 drops of reagent E : leave to act 5 minutes. 11) Rinse in distilled water and dehydrate through ascending alcohols, clear in xylene and mount.
Sites calcium deposits . Black Nuclei .Red
Reagents
Lithium carbonate saturated solution. 30 ml
D) Sodium sulphite solution. 30 ml E) Mayer's
Bio Optica Milano s.p.a.
via San Faustino 58 • I - 20134 Milano
tel. +39 02212713.1 • fax +39 022153000
Warning and
The product must be used exclusively by specialized technical operators.
precaution
The product is classified as hazardous. Read with attention the information written on the label (dangerous symbols, risks and safety phrases). Consult always the safety data sheet where the information about the risks of the preparation, precautionary measures during use, first aid and disposal are available. Do not use if primary packaging is damaged.
Store the preparation at 2-8°C. Keep the containers tightly closed.
Stability
After the first opening, the product is usable until the expiry date, if correctly stored.
Disposal
Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.
References
Armed Forces Institute of Pathology: Laboratory Methods in Histotechnology. Washington D.C. A.F.I.P. 1994; pag. 197.
Cameron GR.The staining of calcium. J Path and Bact 1930; 33: 929-955.
McGee-Russel SM. Histochemical methods of calcium. J Histochem and Cythochem. 1958; 6: 22-42.
Von Kossa J. Ueber die im Organismus kuenstlich erzeugbren Verkakung. Beitr Path Anat 1901; 29:62.
Bio Optica Milano s.p.a.
via San Faustino 58 • I - 20134 Milano
tel. +39 02212713.1 • fax +39 022153000
EDUCATION Ph.D. September 1998. University of California Davis, Department of History B.A January 1992. Smith College. Magna cum laude with high honors in American Studies. EMPLOYMENT 2010- Professor, Department of History, University of Cincinnati 2005-2010 Associate Professor, Department of History, University of Cincinnati 2000-2005 Assistant Professor, Department of History, Univer
A Magyar Pszichiátriai Társaság Közösségi Pszichiátriai és Addiktológiai Szekciója és az Ébredések Alapítvány VIII. KÖZÖSSÉGI PSZICHIÁTRIAI és ADDIKTOLÓGIAI KONFERENCIÁRA, STIGMA, KÖZÖSSÉG, TÁRSADALOM címmel kerül sor, 2009. december 3-án, Budapesten, a TIT Stúdióban (XI. Zsombolyai u. 6.) A konferenciát Prof. Huszár Ilona
VON KOSSA 
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