Journal vol-2-5

Guru/Journal of Engineering, Science and Management Education/Vol. 2, 2010/73-77 MICROBIAL STUDIES ON SOME METAL COMPLEXES
WITH TETRACYCLINE
Pranay Guru*
Received July 22, 2010, Revised July 23, 2010 ; Accepted Aug. 26, 2010 Abstract
RESULTS AND DISCUSSIONS
The present paper deals with the microbial studies of Ni(II) In general all the tested complexes showed higher toxicity and Co(II) complexes with antibiotic drug tetracycline. The against bacterial and fungi under study .
composition Ni (C H N O ) WO 3H O and Co (C H N Antibacterial Activity
O )WO 4H O complexes has been suggested on the basis of From the Tables 1 and 2 it is concluded that complex S1 has elemental analysis and molar conductance values. The exhibits maximum zone of inhibition against Shigella microbial studies of these complexes were studied on flexneri at the concentration of 0.1 M. Even at the pathogenic bacteria using gram +ve (Bacillus subtilis and concentration of 0.01 M it has shown good zone of inhibition Staphylococcus aureus) and gram -ve (Shigella flexneri, in compression to other tested complexes. Against Salmonella typhosa, Escherichia coli) and some fungi Salmonella typhosa good antibacterial activity was observed (Aspergillus flavus, Fusarium oxysporum, Chrysosporium against almost all the tested complexes.
pannicale, Alternaria solani, Candida albicans ). Complex S2 shows maximum zone of inhibition Salmonella Keywords-Tetracycline, Complexes, Microbial Activity, typhosa. Similarly, against Escherichia- coli maximum inhibitory effect were produced by complex S2. Bacillus INTRODUCTION
subtitles was found to be more susceptible against complex The use of raw extract of plants for the amelioration of human S2. Complex S2 found to be more active and shown higher sufferings without knowing their chemical constituent and zone of inhibition against Staphylococcus aureus in active principles is well-known. Such importance of plant comparison to S1. On comparing the anti-bacterial efficacy of products and their utility encouraged human being to find out these complexes, it is concluded that complex S2 gave the mysteries and marvels of biological processes for attaining this aim. It was noticed that several metal complexes For the sake of comparison of the antibacterial properties of with drugs posses more medicinal importance as compared to these complexes the zone of inhibition have been graphically In the present age a large number of common diseases of man Anti Fungal Activity
and animals which are caused by bacterial infections are Study of anti-fungal activity of complexes S1, S2 was carried treated by antimicrobial drug and may require a long out against selected five fungi namely Aspergillus flavus, treatment for their cure. However, the present study shows Candida albicans, Alternaria solani, Fusarium oxysporum that some metal-complexes could be of better use against and Chrysosporium pannicale at varying concentration of these diseases as these complexes can easily be metabolized complexes 0.1M, 0.5 M and 0.01 M respectively and the into man or animal systems along-with their quick curative result are recorded in terms of zone of inhibition which also .In doing so we have carried out microbial studies of includes the diameter of filter paper disc (6mm).
Ni (II) and Co(II) with tetracycline.
From Table 3 it is observed that at the concentration of 0.1M EXPERIMENTAL
the complex S2 shows maximum zone of inhibition against Microbial studies of the synthesized complexes were Aspergillus flavus .Similarly good inhibitory efficacy was performed at Department of Microbiology, Dr H.S. Gour also observed at the same concentration of complexes S2 University Sagar (M.P.) and Govt. Veterinary college against Aspergillus flavus. Against Candida albicans at the Jabalpur (M.P.) using paper disc method against the concentration of 0.1M complex S2 have shown maximum following pathogenic bacteria using gram +ve (Bacillus activity but similarly, considerable zone of inhibition were subtilis and Staphylococcus aureus) and gram -ve (Shigella also recoded in case of complexes . It is evident from Table 3 flexneri, Salmonella typhosa, Escherichia coli) and some that even at the concentration of 0.01 M both the complexes fungi (Aspergillus flavus, Fusarium oxysporum, were found to be active against Candida albicans. Maximum Chrysosporium pannicale, Alternaria solani, Candida zone of inhibition were recorded these complexes at the albicans). The synthesized complexes were screened for the concentration of 0.1M against Alternaria solani. The antibacterial and antifungal activity using standard paper disc complexes at the concentration of 0.5 M have also gave promising results. The complex S2 at the concentration of 0.1 * Department of Engineering Chemistry, People's College of Research & Technology, Bhopal (M. P.) India, pranayguru@rediffmail.com Journal of Engineering, Science and Management Education M produced maximum zone of inhibition against Fusarium Table 3 : Antifungal Activity of Synthesized Complexes
oxysporum. Microorganism Chrysosporium pannicale was Stain of Fungi/
found susceptible against all the complexes tested at their Zone of Inhibition
concentration of 0.1M and 0.5 Complex S2 was found to Concentration
posses good antifungal activity at 0.1 M concentration.
The results are presented in figures 6-10.Antimicrobial Aspergillus flavus
properties of the original drug against selected microorganism were also compared. It could be observed that synthesized complex have shown promising result compared Candida albicans
to commercial original drug tetracycline.
From the present study it is concluded that the complexes Alternaria solani
-
used have shown considerable antimicrobial activity and are found more effective against tested bacteria comparative to fungi. Results also indicate that inhibitory power of the Fusarium
complexes decreases with the increase of their concentration. oxysporum
Table-1 : Complexes of Different Metals Were
Chrysosporium
Marked Has S1 ,S2 as follows
pannicale
Including diameter of filter-paper disc (6mm) H N O ) WO 4H O, S2 =Co (C H N O ) WO 3H O Table-2 : Antibacterial Activity of
Synthesized Complexes
Stain of Bacteria/
Zone of Inhibition
Concentration
Inhibition
Bacteria
Shigella flexneri
Concentration
Salmonella
S1 = Ni (C H N O ) WO 4H O, S2 =Co (C H N O ) WO 3H O typhosa
Graph 1 : Comparative Antibacterial Activity Of
Complexes Against Shigella Flexneri
Escherichia-
coli
Bacillus subtilis
Zone of 15
Inhibition
Staphylococcus
aureus
Concentration
Including diameter of filter-paper disc (6mm) S1 = Ni (C H N O ) WO 4H O, S2 =Co (C H N O ) WO 3H O S1 = Ni (C22 H24 N2 O8) WO4 4H2O, S2 =Co (C22 H24
Graph 2 : Comparative antibacterial activity of
complexes against Salmonella typhosa
Journal of Engineering, Science and Management Education Zone of 10
Inhibition
Inhibition 8
Concentration
Concentration
Graph 3 : Comparative antibacterial activity of
Graph 6 : Comparative antifungal activity of
complexes against Escehrichia-coli
complexes against Aspergillus flavus
Inhibition
Inhibition
Concentration
Concentration
Graph 4 : Comparative antibacterial activity of
Graph 7 : Comparative antifungal activity of
complexes against Bacillus subtilis
complexes against Candida albicans
Inhibition
Inhibition
Concentration
Concentration
Graph 5 : Comparative antibacterial activity of
Graph 8 : Comparative antifungal activity of
complexes against Staphylococcus aureu
complexes against Alternaria solani
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S1 = Ni (C H N O ) WO 4H O,
B. William “A Text Book of Pathology Structure and Function in Disease”, 8 Ed., 1970, 324.
Graph 9 : Comparative Antifungal Activity of
G.S. Willson, and A.A Miles., “Topley and Willson's Complexes Against Fusarium Oxysporum
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Inhibition
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M. K. Kathal, R.K. Gautam, J. Ind. Chem., 67(2), 95, ACKNOWLEDGMENT
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