Redetb.org

Comunicação Breve
Drug resistance in Mycobacterium tuberculosis strains isolated
from sputum samples from symptomatic outpatients –
Complexo de Manguinhos, Rio de Janeiro, Brazil*
Resistência a drogas em cepas de Mycobacterium tuberculosis isoladas
de amostras de escarro de pacientes ambulatoriais sintomáticos –
Complexo de Manguinhos, Rio de Janeiro, Brasil
Joycenea Matsuda Mendes1, Maria Cristina Lourenço2, Rosa Maria Carvalho Ferreira3,
Leila de Souza Fonseca4, Maria Helena Feres Saad5
Abstract
This study aimed to assess drug resistance in Mycobacterium tuberculosis strains isolated from sputum samples. To that end, sputum samples
were collected from 263 patients suspected of having tuberculosis. All subjects lived in the Complexo de Manguinhos, which is located in
the city of Rio de Janeiro, Brazil. Cultures testing positive between October of 2000 and December of 2002 were tested to determine strain
susceptibility to isoniazid, rifampicin, streptomycin, ethionamide, and ethambutol. Of the 75 patients diagnosed with tuberculosis, resistance
to at least one of the drugs was found in 16 (21.4%). Of those 16 patients, 8 (50%) were new cases, and 8 (50%) had previously been
treated. Multidrug-resistant tuberculosis was identified in 8 (10.6%) of the 75 patients, being associated with previous treatment in 6 (8%).
The incidence of multidrug-resistant tuberculosis might have been underestimated, since M. tuberculosis was not isolated from all of the
samples testing positive for acid-fast bacilli. However, at least, our findings shed some light on the problem.
Keywords: Tuberculosis; Drug resistance; Drug resistance, multiple.
Resumo
Para descrever a resistência a drogas em cepas de Mycobacterium tuberculosis isoladas de amostras de escarro de 263 pacientes suspeitos de
tuberculose moradores do Complexo de Manguinhos, Rio de Janeiro, Brasil, as culturas positivas entre outubro de 2000 e dezembro de 2002
foram submetidas a teste de sensibilidade para isoniazida, rifampicina, estreptomicina, etionamida e etambutol. Resistência a qualquer das
drogas foi encontrada em 21,4% (16/75) dos pacientes diagnosticados com tuberculose. Destes, 50% (8/16) eram casos novos e 50% (8/16)
eram casos com tratamento anterior. A tuberculose multirresistente foi encontrada em 10,6% (8/75) do total de pacientes, estando associada
a tratamento anterior em 8% (6/75) deles. Nossos resultados podem ter sido subestimados, pois M. tuberculosis não pôde ser isolado em
todas as amostras positivas para bacilos álcool-ácido resistentes. Contudo, eles pelo menos revelam parte do problema.
Descritores: Tuberculose; Resistência a drogas; Resistência a múltiplas drogas.
* Trabalho realizado no Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, Fundação Osvaldo Cruz – FIOCRUZ – Rio de Janeiro (RJ) Brasil.
1. Mestre em Clínica Médica. Faculdade de Medicina da Universidade Federal do Rio de Janeiro – UFRJ – Rio de Janeiro (RJ) Brasil.
2. Pesquisadora Titular Chefe do Serviço de Bacteriologia. Instituto de Pesquisa Clínica Evandro Chagas – IPEC – Rio de Janeiro (RJ) Brasil.
3. Mestre em Ciências (Microbiologia) no Instituto de Microbiologia. Universidade Federal do Rio de Janeiro – UFRJ – Rio de Janeiro (RJ) Brasil.
4. Professora Titular. Universidade Federal do Rio de Janeiro – UFRJ – Rio de Janeiro (RJ) Brasil.
5. Pesquisadora Titular do Laboratório de Microbiologia Celular. Instituto Oswaldo Cruz – FIOCRUZ – Rio de Janeiro (RJ) Brasil.
Endereço para correspondência: Maria Helena Féres Saad. Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil, 4365, CEP 21045-900, Rio de Janeiro, RJ, Brasil.
Tel. 55 21 2598-4346. Fax. 55 21 2270-9997. E-mail: saad@ioc.fiocruz.brRecebido para publicação em 7/11/2006. Aprovado, após revisão, em 6/2/2007.
Mendes JM, Lourenço MC, Ferreira RMC, Fonseca LS, Saad MHF Tuberculosis (TB) is the leading cause of mortality culture and susceptibility tests are not part of the from infectious diseases worldwide, accounting for routine of the health care laboratory, they were approximately 2 million deaths each year, especially performed in the Mycobacteriology Laboratory of in developing countries.(1,2) Despite the existence the FIOCRUZ-operated Evandro Chagas Research of effective chemotherapy and the widespread use Institute. The M. tuberculosis strain isolates were of the bacillus Calmette-Guérin (BCG) vaccine, the tested on Löwenstein-Jensen medium using the disease has never been properly controlled in these standard proportion method for susceptibility to isoniazid (INH), rifampicin (RIF), streptomycin (SM), Brazil ranks fifteenth in the world in the number ethionamide (ETH) and ethambutol (EMB). An of estimated cases, with an incidence rate of isolate was defined as resistant when the percentage 62/100,000 inhabitants.(3) Higher incidence rates of colonies exceeded 1% of the growth on a drug- are seen in densely populated cities such as Rio free medium (control) at the critical concentrations de Janeiro (114/100,000), which has the highest of 0.2, 40, 4.0, 20, and 2.0 µg/mL, respectively.(7,8) TB-related mortality rate (6.53/100,000).(4,5) Multidrug resistance (MDR) was defined as resis- The TB situation can be even worse in areas with tance to at least INH and RIF. All TB cases diagnosed socially vulnerable populations. One such area is the in the Complexo de Manguinhos were treated and Complexo de Manguinhos, a slum located in the city monitored at the CSEGSF/ENSP/FIOCRUZ.
of Rio de Janeiro. Although the efforts made by the Centro de Saúde Escola Germano Silval Faria (CSEGSF, 2002, sputum samples from 263 suspected cases of Germano Sinval Faria School Health Center), working TB were analyzed by culture, and 80 of them were in collaboration with the Escola Nacional de Saúde confirmed as cases of TB. However, 5 samples were Pública (ENSP, National School of Public Health) contaminated, and therefore only 75 samples were of the Fundação Oswaldo Cruz (FIOCRUZ, Oswaldo available for susceptibility testing. The mean age of Cruz Foundation) have effectively lowered the TB the patients was 36 ± 14 years (range, 18-77 years), incidence rate in the Complexo de Manguinhos,(6,7) and the majority of the patients were male it remains high: 275, 260, and 235/100.000 in 1997, 1998, and 1999, respectively – data obtained (p = 0.04). Serology for HIV was available for 66 of from the CSEGSF/ENSP/FIOCRUZ Programa de the patients, 4 (6%) of whom were HIV-positive. Controle da Tuberculose (PCT, Tuberculosis Control Data were unavailable for one patient, who was Program). However, drug resistance testing has reported to be in prison. never been performed. The objective of this study As shown in Table 1, 59 (78.7%) of the 75 patients was to describe drug-resistance in Mycobacterium were infected with strains susceptible to all drugs tuberculosis strains isolated from adult individuals tested: 55 were new TB cases, and 4 were previously with respiratory symptoms and suspected of having treated TB cases. The overall rate of resistance was TB (cough for 3 weeks) who sought treatment at 21.3%: 12.7% of the new cases and 66.7% of the the CSEGSF/ENSP/FIOCRUZ between October of previously treated cases. Strains presenting mono-or 2000 and December of 2002.
multiple-resistance to SM were found to infect The Complexo de Manguinhos comprises mainly new cases (4 of the 6 cases, 5.3% of the 12 communities with slum-like conditions. However, sample), and no strains showing mono-resistance to there are two exceptions, the communities designated RIF or ETH were detected. As expected, most of the CHP2 and Vila Turismo, which have large demogra- patients infected with MDR strains (6 of the 8 cases, phic populations and partial sanitation infrastructure 8% of the sample) had been previously treated, with treated water and a sewage system. Overall, the none were HIV-positive, and 2 (2.6% of the sample) Complexo de Manguinhos has 42,100 inhabitants were new cases, together accounting for 10.6% of distributed in approximately 8,000 houses (Rio de the sample. Of the 8 resistant new cases, 3 were Janeiro Institute for State Management Planning). found in the largest community in the Complexo de Physical examinations were carried out by the PCT Manguinhos, the CHP2; and MDR cases were found staff at the CSEGSF/ENSP/FIOCRUZ, at which time in two communities, Vila Turismo (7324 inhabitants) sputum specimens were obtained and tested for and Ex-Combatentes (934 inhabitants), although acid-fast bacilli (Ziehl-Neelsen staining).(7) Since resistant strains infecting previously treated patients Drug resistance in Mycobacterium tuberculosis strains isolated from sputum samples from symptomatic outpatients – Complexo de Manguinhos, Rio de Janeiro, Brazil Table 1 - Susceptibility testing of Mycobacterium tuberculosis strains isolated from tuberculosis patients (new and
previously treated cases) living in the Complexo de Manguinhos, Rio de Janeiro, Brazil, between October of 2000 and
December of 2002.
INH: isoniazid; RIF: rifampicin; EMB: ethambutol; and SM: streptomycin.
are more commonly found in communities with Mozambique.(10) The high incidence of drug-resistant more than 4000 inhabitants.
strains in Mozambique reflects a pattern found in The overall rate of resistance in the Complexo de TB/HIV co-infected patients, who are more suscep- Manguinhos was 21.4%, and, although the number tible to TB infection, and, according to the authors, of strains tested was small, the importance of this is associated with a high risk of exposure to resis-datum is that, in addition to being the first time tant strains due to failure on the part of the PCT. In that resistance was determined in the community the Complexo de Manguinhos, most of the patients studied, it was obtained in a high-burden, limited- were HIV-negative, and the resistance problem resource community (in Rio de Janeiro, Brazil) where, seemed to be related to treatment non-compliance, despite the existence of a public health care facility as indicated by the high incidence of MDR strains that preferentially serves this community, TB inci- infecting previously treated patients. In addition, dence rates remain a cause for concern.(9) The rate although the study population comprised only of mono-resistance to INH and SM obtained in this 36.4% of all TB patients diagnosed at the time, study was high. However, the SM mono-resistance, this situation might reflect the reality in the area. unlike the INH mono-resistance, which was more With regard to resistant strains infecting new cases, frequent among previously treated cases, was primary resistance should be strongly suspected in detected among new cases (Table 1). Similar results at least one patient, since his brother was under were obtained in TB/HIV co-infected patients in treatment for MDR-TB presenting the same resis-Mozambique.(10) This is a concern since treatment tance pattern, although molecular typing was not regimen I, which is recommended for untreated performed to confirm the genetic identity of the patients, does not contain SM, suggesting that older strains. Other factors might be involved in the resis-strains (related to endogenous reactivation) continue tance situation in the Complexo de Manguinhos. to circulate in the Complexo de Manguinhos. The One such factor is that, during the study period, number of HIV-positive patients in our sample was the PCT was closed or worked poorly because of low (6%), and the mean rate of TB/HIV co-infection the violence perpetrated by organized crime gangs in the study period was 16.3%.(9) This might have in the region.
created a bias in our results, since the isolates for This study is the first to provide information regarding drug resistance in the limited-resource The rate of MDR-TB in the Complexo de area of the Complexo de Manguinhos, and the Manguinhos was similar to that found in results show that the incidence of resistant strains Mendes JM, Lourenço MC, Ferreira RMC, Fonseca LS, Saad MHF isolated from new and previously treated cases is 2. Goldrick BA. Update: Tuberculosis in the United States: the high. The highest incidence of resistance in new CDC updates its guidelines, as infection rates decline slowly. Am J Nurs. 2005;105(7):85-6.
cases was found in the most densely populated 3. World Health Organization. Global tuberculosis control. community (CHP2: 8655 inhabitants), and MDR-TB was spread among previously treated patients living 4. Hijjar MA. Tuberculose: desafio permanente. Cad Saúde in poor and disorganized communities with a high 5. Secretaria de Saúde e Defesa Civil do Estado do Rio de population density, such as Mandela de Pedra, Janeiro [homepage on the Internet]. Rio de Janeiro: [cited Nelson Mandela, and Parque Oswaldo Cruz, lending 2004 Nov 1]; Available from: http://www.saude.rj.gov.br/ credence to the assumption that crowded areas 6. Mendes JM, Santos MO, Esteves MA, Saad MHF, Patroclo and low socioeconomic conditions favor the spread MA. Aspectos epidemiológicos da tuberculose no Complexo of resistant strains. However, the incidence might de Manguinhos, Rio de Janeiro, Brasil. Estudo retrospectivo have been underestimated, since M. tuberculosis no período de 1986 a 1994. Pulmão RJ. 2002;11(2):46-50.
7. Brasil. Ministério da Saúde. Fundação Nacional de Saúde. was not isolated from all the samples testing posi- Centro de Referência Prof. Hélio Fraga. Sociedade Brasileira tive for acid-fast bacilli during the study period. de Pneumologia e Tisiologia. Controle da tuberculose: uma We worked with a sample of patients with chronic proposta de integração ensino-serviço. 5ª Ed. Rio de Janeiro: FUNASA/CRPHF/SNPT, 2002.
cough treated at one CSEGSF clinic, and the resis- 8. Canetti G, Fox W, Khomenko A, Mahler HT, Menon NK, tance pattern obtained represents the situation in Mitchison DA, et al. Advances in techniques of testing this group (34.6% of all patients diagnosed during mycobacterial drug sensitivity, and the use of sensitivity the study period). Nevertheless, our results reveal, at tests in tuberculosis control programmes. Bull World Health Organ. 1969;41(1):21-43.
least to some extent, the problem in the Complexo 9. Mendes JM, Fonseca LS, Lourenço MC, Ferreira RMC, Saad de Manguinhos, clarifying it so that it can be MHF. Retrospective study: tuberculosis epidemiological aspects in Complexo de Manguinhos an urban slum area in Rio de Janeiro, Brazil, 2000-2002. J Bras Pneumol. 2007;33(4):443-7.
References
10. Nunes EA, De Capitani EM, Coelho E, Joaquim OA, Figueiredo IR, Cossa AM, et al. Patterns of anti-tuberculosis 1. World Health Organization. Anti-tuberculosis Drug Resistance drug resistance among HIV-infected patients in Maputo, in the world. Report Nº 2. Prevalence and Trends. WHO/CDS/ Mozambique, 2002-2003. Int J Tuberc Lung Dis.

Source: http://www.redetb.org/attachments/1333_2007_33_5_14_english.pdf

icp.hacettepe.edu.tr

Applications for LA-(MC)-ICP-MS (text need some serious improvement) Stable isotopes: Li The two isotopes of lithium (7.5% of 6Li and 92.5% of 7 Li) have a relative mass differenceof approximately 16.6% (larger than B with 10% and 16O-18O with 12.5%). Hence Li isotopicfractionation seems to be a promising tool to monitor low surface chemical processes. Lithiumisotopic composition v

Chart 6.doc

The Feasts of Israel - Chart The Four Trees of the Feasts of Tabernacle Leviticus 23:40 - And ye shall take you on the first day the boughs of goodly trees, branches of palm trees, and the boughs of thick trees, and willows of the brook; and ye shall rejoice before the Lord your God seven days. Type of Tree Fruit- Use Of Tree (Hebrew Nick-Name

© 2010-2017 Pharmacy Pills Pdf