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Hplc columns

310-323_HPLCcol.qxp 11/19/2008 10:05 AM Page 310 HPLC COLUMNS
Allure® Columns: 5μm particles; 60Å pore size
Small pore size in a high-purity, Type B silica provides a large surface area. High carbon
loads, highly retentive. An excellent choice for light scattering (ELSD) and MS detectors,
in which more organic solvent in the mobile phase gives better sensitivity.
Allure® C18 Columns (USP L1)
Excellent Columns for LC/MS and ELSD
Physical Characteristics:

Chromatographic Properties:
Most retentive of our alkyl stationary phases due to large surface area of the base silica andhigh-density bondings. Provides excellent peak shapes for a wide range of compounds.
Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page # Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .526 Herbicides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .504 Pesticides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .506 Allure® Basix Columns (USP L10)
Excellent Columns for LC/MS and ELSD
Physical Characteristics:

Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page # Chromatographic Properties:
Acetaminophen, Narcotic Analgesics . . . . . . . . . . . . .519 Highly retentive propyl cyano phase. Excellent choice for basic compounds and for analytes Albuterol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .528 containing amine group functionality.
Antiarrhythmics . . . . . . . . . . . . . . . . . . . . . . . . . .521, 522 Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .526 Antidepressants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .527 Herbicides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .503 Ibuprofen & Pseudoephedrine . . . . . . . . . . . . . . . . . .533 Sedatives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .532 Steroids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .530 looking for more
chromatograms?
310-323_HPLCcol.qxp 11/19/2008 10:05 AM Page 311 HPLC COLUMNS
Allure® PFP Propyl Columns (USP L43)
Excellent Columns for LC/MS and ELSD
Physical Characteristics:

Chromatographic Properties:
A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, halogenatedcompounds, β-blockers, and tricyclic antidepressants.
Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page # Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .524 Benzodiazepines . . . . . . . . . . . . . . . . . . . . . . . . . . . . .498 Catecholamines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .494 Cocaine, Ecgonine Methyl Ester . . . . . . . . . . . . . . . . .497 Nucleic Acid Bases . . . . . . . . . . . . . . . . . . . . . . . . . . .493 Opiates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .496 System Suitability Mix . . . . . . . . . . . . . . . . . . . . . . . . .499 Allure® Aqueous C18 Columns (USP L1)
Excellent Columns for LC/MS and ELSD
Physical Characteristics:

pH range: 2.5 to 7.5temperature limit: 80°C Chromatographic Properties:
Highly retentive and selective phase for separating polar analytes, including polar acidic compounds. Compatible with highly aqueous (up to 100%) mobile phases. Highly base deactivated. An excellent choice when analyzing a wide range of compounds, as in LC/MSscreening methods.
ordering note
To order a 2.1mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add “-700” to thecatalog number for the column. Example: 100mm x 4.6mm ID Ultra C18 column with Trident Integral Inlet Fitting: 9174315-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 327.
available
For guard cartridges for these columns, see page 329.
310-323_HPLCcol.qxp 11/19/2008 10:05 AM Page 312 HPLC COLUMNS
Allure® Biphenyl Columns (USP L11)
Physical Characteristics:

Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page # Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .526 Cannabinoids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .496 Chromatographic Properties:
Corticosteroids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 Highly retentive and selective for aromatic and unsaturated compounds. Increased retention NSAIDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .531 and selectivity, compared to phenyl phases. Excellent selectivity for steroids, tetracyclines,explosives, and other unsaturated compounds.
Steroids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .529 Download your free copyfrom www.restek.com.
Allure® Organic Acids Columns
Physical Characteristics:

pH range: 2.5 to 7.5temperature limit: 80°C Chromatographic Properties:
Allure® Organic Acids columns provide enhanced retention and selectivity for polar organicacids, allowing the separation to be performed on a single 30cm column. An Allure® OrganicAcids column effectively resolves key organic acids such as tartaric and quinic acids, using thechromatographic conditions specified in AOAC method 986.13. Retention is stable and repro-ducible, even with the 100% aqueous mobile phase specified in the AOAC method.
Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page # Carboxylic Acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .514 Fruit Juice Acids . . . . . . . . . . . . . . . . . . . . . . . . .514, 515 Note: Other dimensions available on request.
ordering note
To order a 2.1mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add “-700” to thecatalog number for the column. Example: 100mm x 4.6mm ID Ultra C18 column with Trident Integral Inlet Fitting: 9174315-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 327.
For guard cartridges for these columns, see page 329.
310-323_HPLCcol.qxp 11/19/2008 10:05 AM Page 313 HPLC COLUMNS
Allure® Silica Columns (USP L3)
Physical Characteristics:

pH range: 2.5 to 7.5temperature limit: 80°C Chromatographic Properties:
Highly retentive phase for normal phase separations. Very high surface area, Type B silicapacking.
Allure® AK Columns
Physical Characteristics:
pH range: 2.5 to 7.5temperature limit: 80°C Chromatographic Properties:
This highly retentive, highly selective phase, unique to Restek, was developed specifically forthe analysis of aldehydes and ketones as DNPH derivatives. Allure® AK is a reversed phaseHPLC material that has the unique ability to separate all thirteen carbonyl compounds speci-fied in California Air Resources Board (CARB) Method # 1004, using a simple acetonitrile/water gradient, in less than 15 minutes. Other columns require long analysis times or the useof tetrahydrofuran.
Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Page # 5µm Columns with Trident Integral Inlet Fittings Carbonyls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .500 Carbonyls by CARB Method 1004 on an Allure® AK column.

Source: http://www.teknokroma.es/UserFiles/HPLC/Allure.pdf

Microsoft word - basic theory_karl fischer titration

Karl Fischer Titration What is a Karl Fischer chemical reaction? I2 + 2H2 O+ SO2 -> 2HI + H2SO4 This reaction taking place in the presence of a base and a solvent. (a typical solvent could be methanol, and a base imidazole) How does the Karl Fischer determination work? A chemical reaction takes place between Iodine (I) and water (W) with the reactants being in a 1 to

Microsoft word - usmle 2ck practice questions england.doc

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