Medicamentsen-ligne vous propose les traitements dont vous avez besoin afin de prendre soin de votre santé sexuelle. Avec plus de 6 ans d'expérience et plus de 90.000 clients francophones, nous étions la première clinique fournissant du acheter cialis original en France à vente en ligne et le premier vendeur en ligne de Kamagra dans le monde. Pourquoi prendre des risques si vous pouvez être sûr avec Medicamentsen-ligne - Le service auquel vous pouvez faire confiance.

Codex-non-wash calcium dye kitl

Technical Data Sheet Codex ACTOne™ Non-Wash Calcium Dye Kit Product Information Catalog Number: Codex Calcium Dye, 10 vials, lyophilized (Part No: 80500-110) 10X Calcium Dye Signal Enhancer, 10 ml (Part No: 80500-112) Description The Codex ACTOne™ Non-Wash Calcium Dye Kit allows homogeneous measurement of intracellular calcium changes caused by activation of G-protein coupled receptors or calcium channels. The assay involves only one step of dye addition and does not require any washing steps. It is user friendly and cost effective. The assay can be easily implemented in a high throughput environment. Storage Codex Calcium Dye Codex Biosolutions, Inc. 401 Professional Drive, Suite 160, Gaithersburg, MD 20879 Tel: (240)-632-8810; Fax: (240)-632-8820 E-Mail: orders@codexbiosolutions.com http://www.codexbiosolutions.com/ 1. Seed 80 µl of cell suspension into each well of a 96-well plate or 20 µl of cell suspension into each well of a 2. Grow the cells overnight in a CO2 incubator 1. Prepare Buffer A (1X HBSS with 20 mM HEPES): 10 ml of 1M HEPES, pH 7.3 + 490 ml of 1X HBSS Dissolve 142 mg of Probenecid in 1 ml of 1N NaOH 3. Prepare stock solution of calcium dye Add 8µl of DMSO into each well containing 50 µg of calcium dye 4. Prepare 2X Dye Loading Buffer (1 plates). Add 0.8 ml of Codex 10X Calcium Dye Signal Enhancer into 7.2 ml of Buffer A. Add 8 µl of calcium dye stock solution. Mix well by vortexing. 1. Take the cell plate out from the incubator. 2. Add same volume of 2X Dye Loading Buffer into each well, 80 µl to a 96-well plate or 20 µl to a 384-well 3. Incubate at 37 °C incubator for 1 hr. 4. Take the cells out of the incubator and leave at room temp (in the dark) for 30 min. 5. Put the plate into the instrument for assay For assays performed on a FlexStation (MDS), use the following wavelength parameters. Excitation: 485 nm; Emission: 530 nm; Cutoff 515 nm Note. Dispense speed and height for compound additions need to be optimized for each instrument. Codex Biosolutions, Inc. 401 Professional Drive, Suite 160, Gaithersburg, MD 20879 Tel: (240)-632-8810; Fax: (240)-632-8820 E-Mail: orders@codexbiosolutions.com http://www.codexbiosolutions.com/ Figure 1. Response of endogenous P2Y receptors to ATP. HEK293 cells were plated overnight in 20 µl culture medium on a 384 well Biocoat poly-D lysine coated plate. The next day, the cells were dye-loaded by adding 20 µl of 2X Dye Loading Buffer and incubating for 1 hour at 37°C. ATP solution was added (10 µl/well) by a FLIPR Tetra (Molecular Devices), and the data was recorded simultaneously. A. Kinetic curve of calcium response to different concentrations of ATP. B. ATP dose response curve (n = 4). EC50 = 1.1 µM. Limited Label License The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product for research use only by the buyer, where such research does not involve testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data to third parties; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For more information, contact Business Development, Codex Biosolutions, Inc. 401 Professional Drive, Suite 160, Gaithersburg, MD 20878, USA. Tel: (240)-632-8810. Fax: (240)-632-8820 Codex Biosolutions, Inc. 401 Professional Drive, Suite 160, Gaithersburg, MD 20879 Tel: (240)-632-8810; Fax: (240)-632-8820 E-Mail: orders@codexbiosolutions.com http://www.codexbiosolutions.com/

Source: http://codexbiosolutions.com/wp-content/uploads/2012/01/Codex-non-wash-calcium-dye-kit.pdf

Microsoft word - other types of hiv support.docx

Other Types of HIV/AIDS Support The data in this report represent financial contributions only from HIV/AIDS funders, in the form of external grants and programs. Such financial contributions can be used to conduct a trend analysis because they are quantifiable as monetary amounts and are measurable in a clear and distinct way. However, many funders contribute in other important ways that

Untitled

for centuries are still central to the national identity of we shall be opening a Europe House in Ashgabat. modern-day Turkmens. You can clearly recognize this A further stimulus to our relations is the enormous in our government’s foreign policy based on the prin-potential the EU has in economics and trade, fi nance ciple of positive neutrality, which has been followed and investment,

Copyright © 2010-2014 Pharmacy Pills Pdf